O-Linked neutral sugar chains of porcine zona pellucida glycoproteins
1993
Hirano, T. | Takasaki, S. | Hedrick, J.L. | Wardrip, N.J. | Amano, J. | Kobata, A.
The O-linked sugar chains were liberated from porcine zona pellucida glycoproteins by alkaline-borohydride treatment and the structures of neutral oligosaccharides were determined. The major oligosaccharides had the following structures with type-1 cores, (Galbeta 1 leads to 4TGlcNAcbeta 1 leads to 3)1-3-Galbeta 1 leads to 3N-acetylgalactosaminitol (54%) and GLcNAcbeta 1 leads to 3(Galbeta 1 leads to 4GlcNAcbeta 1 leads to 3)0-1Galbeta 1 leads to 3N-acetyl-galactosaminitol (16%). Approximately 6% of the oligosaccharides had the structure with a type-3 core, Galbeta 1 leads to 4GlcNAcbeta 1 leads to 3N-acetylgalactosamintol. Oligosaccharides having alpha-galactose (2 %) and beta-GalNAc (4%) at their non-reducing termini were also found as minor components.
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