Accumulation of prolamines and glutelins during rice seed development: a quantitative evaluation
1993
Li, X.X. | Okita, T.W.
The levels of prolamines and glutelins, the storage proteins of rice, were quantified during seed development by immunoblot analysis. Although both storage proteins were first detected in 10 day old seeds and their amounts steadily increased throughout seed development, the relative proportions of glutelins and prolamines were not constant. The molar ratio of glutelins to prolamines was 1.7 in 10 day old seeds and this ratio steadily decreased to 1.2 in 25 day old seeds due to the increased synthesis and accumulation of prolamines specifically during the latter stages of seed development. In vivo pulse chase labelling studies confirmed that the rate of prolamine synthesis as compared to that evident for glutelin increased during the latter half of seed development and that protein turnover was not the basis for the differences in accumulation patterns of these storage proteins. These results indicate that the storage proteins exhibit different temporal accumulation patterns during seed development and, moreover, demonstrate that prolamines comprise a much larger proportion of the total storage protein fraction than previously recognized.
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