First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina
Carbonari, Claudia C. | Deza, Natalia | Flores F.-De Mêge, Mario (Flores Figueroa-De Mêge) | Gasparini, Alejandra | Manfredi, Eduardo | Rivas, Marta
We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx₁ and stx₂). A mPCR to detect the rfbO₁₀₄, fliCH₄ and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO₁₁₃ genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO₁₁₃, rfbO₁₀₄, fliCH₄ and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes.
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