Prolactin-stimulated polymerization of acetyl-CoA carboxylase in explants of mid-pregnant rat mammary gland
2001
Oben, J.E. | Dils, R.R.
Prolactin significantly increased the rate of fatty acid synthesis in explants of mid-pregnant rat mammary gland cultured for 96 h with insulin plus corticosterone. Under these conditions, prolactin increased the specific activity of total acetyl-CoA carboxylase in nuclear-free homogenates of explants by 2.6, and increased the proportion of the enzyme in the active polymeric form from 0.44 to 0.89. Removal of prolactin after 48 h in culture decreased the specific activity of the total enzyme by about half, and decreased the proportion as polymer to 0.52. The results show that prolactin plays a major role in mid-pregnant rat mammary gland in the polymerization which accompanies increased activity of the total enzyme and increased rate of fatty acid synthesis.
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