Composition and quantitation of saponins in alfalfa (Medicago sativa L.) seedlings
1998
Oleszek, W.A.
The concentrations of individual saponins in germinating alfalfa seeds and seedlings between 1 and 16 days of growth were determined by HPLC. It was shown that monodesmosidic medicagenic acid glycoside was synthesized after 4 days of germination and subsequently followed by bidesmosidic saponin production. The total saponin concentration increased from 2.12 micromole/g of dry matter at the beginning of germination to around 6 micromole/g after 8-16 days of seedling growth. It was concluded that previous reports on saponin concentration in alfalfa seedlings (8-10% in dry matter) as measured by biological tests were highly overestimated; the real concentration is several times lower.
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