Investigation of hemagglutinating activity in seeds of various Trichosanthes species (Family Cucurbitaceae) and comparison of lectins isolated from seeds and tubers of Trichosanthes kirilowii
1993
Dong, T.X. | Ng, T.B. | Wong, R.N.S. | Yeung, H.W. | Xu, G.J.
Potent hemagglutinating activity was detected in seed extracts of 13 out of the 14 Trichosanthes species examined. T pedata seed extract demonstrated the lowest hemagglutinating activity. D(+) galactose, D(+) galactosamine, D(+) raffinose and D(+) fucose but not L(-) fucose inhibited the hemagglutinating activity of these seed extracts. Lectin was purified from Trichosanthes kirilowii seed extract by a procedure involving solvent fractionation. gel filtration on Sephadex G-75 and affinity chromatography on Lacto-Sepharose. The biochemical properties of T. kirilowii seed lectin were compared with those of T. kirilowii tuber isolectins. Their sugar binding specificities and amino acid compositions were similar. However, there was a difference in molecular weight.
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