In vivo and in vitro phytochemical studies of arnica Montana species from Romanian eastern Carpathians
2012
Stefanache, C. | Danila, D. | Necula, R. | Gille, E. | Vantu, S.
Arnica Montana is a rare medicinal plant with traditional use in Europe and North America. Modern cultivation technologies (conventional and non-conventional) support the conservation strategies of the species. This paper presents a comparative analysis of plant material obtained in vivo and in vitro (micropropagation, callus culture and submerged culture). For in situ and ex situ samples, the material consisted in aerial and underground parts, while the material from in vitro cultures was represented by plantlets, callus and cell suspensions. For in vitro cultures we used Murashige and Skoog (MS, 1962) basal medium, tissue culture beeing supplemented with benzil-amino-purine (BAP), and callus cultures with BAP, kinetin (6-furfuryl aminopurine) and 2,4-dichlorophenoxyacetic acid. These media were solidified with different amounts of agar. The cell cultures were maintained in flasks with MS liquid medium, placed on a shaker. Phytochemical analysis of absolute methanol and dichloromethane extracts, respectively, were performed by Thin Layer Chromatography and RP-HPLC-UV (Agilent 1200 series system). They revealed the presence of terpenoids, phytosterols and polyphenolic compounds which varied quantitatively and qualitatively in the experimental samples. As a general trend, the maintenance of biosynthetic capacity of the compounds analyzed was observed.
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