Control of lipase-mediated glycerolysis reactions with butteroil in single liquid phase media with 2-methyl-2-propanol
1993
Yang, B. | Harper, W.J. | Parkin, K.L.
Factors affecting the progress of glycerolysis reactions with butteroil mediated by two lipase preparations (from Pseudomonas sp.) in single liquid phase mixtures containing 2-methyl-2-propanol were evaluated. Within the range of parameters evaluated, the conditions best supporting the formation of monoacylglycerols (MAG) were 25-40% butteroil substrate, 10-20 mg of enzyme/mL of substrate mixture, 35 degrees C, and 100-150 mg of glycerol/mL of substrate mixture (equivalent to a molar ratio of fatty acyl groups to glycerol of 0.61-0.76). Under these conditions, the percent yield of MAG formation from butteroil was 50-60 % (mass fraction) relative to the other acylglycerol species and fatty acids accumulated. These conditions were somewhat dependent on the source of enzyme and probably related to the water content of the enzyme reagents. At temperatures greater than 35 degrees C, and at the greatest levels of glycerol (150 mg/mL) used, a severe restriction of activity was noted, particularly for the lipase preparation with the lesser water content. However, activity and percent yields of MAG could be restored to near-optimal levels by the simple addition of water of up to 1 % of the reaction volume. The ability of the chosen lipases to mediate glycerolysis reactions with butteroil, and other oils, appears to be principally controlled by water availability in the reaction mixture.
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