Development of a quinoxaline-based fluorescent probe for quantitative estimation of protein binding site polarity
2011
Kudo, Kentaro | Momotake, Atsuya | Kanna, Yoko | Nishimura, Yoshinobu | Arai, Tatsuo
The compound 2-[(1E)-2-(1H-pyrrol-2-yl)ethenyl]-quinoxaline (PQX) is a promising fluorescent chromophore for the estimation of protein binding site polarity, due to its full-color solvatochromic fluorescence. A linear relationship was obtained between the peak emission wavenumber and ENT (normalized solvent polarity). The BSA binding site polarity was estimated from the solvatochromic plot.
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