Pigments content in the leaves of lentil under the action of biological preparations

The article presents the results of the field experiment on the influence of microbial preparation (Rhizobium leguminosarum biovar viceae strain K-29, w. s., 100 ml/ha seeds norm) and plant growth regulator (Regoplant, w. s: 250 ml/t – pre-sowing seed treatment; 50 ml/ha – post-germination application) on the content of photosynthetic pigments (chlorophylls a and b, their sum and carotenoids) in the leaves of lentil of Linza variety. The analysis of a and b chlorophylls content, their sum and carotenoids in the leaves of lentil was carried out at bud-formation stage and the beginning of blossoming in selected samples of leaves in the field conditions according to the methods, described by V.F. Havrylenko and T.V. Zhyhalova using spectrophotometer LEKI SS1104. According to the results of the experiments was found dependence of the investigated pigments content in lentil leaves on weather conditions, application of the biological preparation and on phase of growth and development of the culture. Thus, under combined application of biological preparations in the leaves of lentil of Linsa variety there was the increase in the content of chlorophylls a and b, their sum and carotenoids, which created favorable conditions for the main physiological and biochemical processes that account for the formation of high productivity of crops. In the phase of bloom beginning of the lentil, when the activity of growth processes was the highest, content of the investigated pigments was much higher than in the phase of budding. The highest content of pigments in the leaves of lentil was recorded in the variants of the experiment under pre-sowing treatment of seeds with the mixture of microbial preparation (Rhizobium leguminosarum biovar viceae strain К-29, w. s., 100 ml/hа seed rate) and plant growth regulator Regoplant, w. s., 250ml/t with its further post-germination application at the rates 50ml/ha, where the exceed compared to control for the chlorophylls a and b on average made up 35 %, and for carotenoids 43 %.