Characterization by 1H NMR spectroscopy of enzymically derived oligosaccharides from alkali-extractable wheat-flour arabinoxylan
1992
Gruppen, H. | Hoffmann, R.A. | Kormelink, F.J.M. | Voragen, A.G.J. | Kamerling, J.P. | Vliegenthart, J.F.G.
Oligosaccharides obtained from alkali-extractable wheat-flour arabinoxylans by digestion with endo- (1 leads to 4)-beta-D-xylanase from Aspergillus awamori were fractionated by size-exclusion chromatography on Bio-Gel P-2 followed by high-performance anion-exchange chromatography, and subjected to monosaccharide analysis and 1H NMR spectroscopy. The results revealed (1 leads to 4)-linked beta-D-xylopyrano-oligosaccharides partly 3- and/or 2,3-substituted with single alpha-L-arabinofuranosyl groups. The structures of 12 such arabinoxylan oligosaccharides were established.
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