Tetracycline-inducible gene expression system in Leishmania mexicana
2014
Kraeva, Natalya | Ishemgulova, Aygul | Lukeš, Julius | Yurchenko, Vyacheslav
Here we present a T7-driven, tetracycline-inducible system for protein expression in human pathogen Leishmania mexicana. The gene expression in this strain is tightly regulated and dose- and time-dependent. This system can be widely used by the parasitology community to analyze effects of genes of interest on biology, physiology and virulence of parasites causing cutaneous leishmaniases.
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