Ineffectiveness of hot acid sprays to decontaminate Escherichia coli O157:H7 on beef
Brackett, R.E. | Hao, Y.Y. | Doyle, M.P.
The efficacy of warm (20 degrees C) and hot (55 degrees C) acetic, citric and lactic acid sprays on survival of Escherichia coli 0157:H7 on raw beef was determined. Fresh, raw beef sirloin tips were sliced into ca. 1-cm slices with a sanitized slicer. Discs (5-cm diameter, ca. 25-g) were then taken from internal areas of each slice and inoculated with a 5-strain mixture of E. coli O157:H7 such that final populations on beef samples were 10(3) or 10(6) CFU/g. After standing for 15 min, each inoculated disc was sprayed with 1 ml of 0, 0.5, 1.0 and 1.5% of each acid at 20 or 55 degrees C using an atomizer. Acid-treated meat samples were allowed to dry for 15 min and held at 5 degrees C for up to 13 days in sterile plastic pouches. Escherichia coli O157:H7 was acid tolerant, and acids did not differ appreciably in their lack of antimicrobial activity on E. coli O157:H7. For example, reductions in populations differed by < 0.3 log10 CFU/g immediately after treatment and < 0.5 log10 CFU/g after 13 days incubation, compared to untreated controls. None of the acid treatments appreciably reduced E. coli O157:H7 on beef samples nor were any of the acid treatments judged effective for practical uses.
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