Enumeration of bacteria in pure cultures and in foods using a commercial flow cytometer
1991
Patchett, R.A. | Back, J.P. | Pinder, A.C. | Kroll, R.G.
Direct microscopic detection of bacteria in foods can suffer from interference by particulate matter. Flow cytometry enables multiparameter counting of bacteria based on light scattering by cells and fluoresence and this could facilitate the discrimination of bacteria from background material. Use of the Skatron Argus 100 flow cytometer enabled the counting of pure cultures of bacteria to <10(3) cfu ml-1 within a few minutes. Application of the technique to meat samples gave a good correlation with plate counts and enabled enumeration down to at least 10(5) cfu g-1 within minutes. However, with samples of pate and milk, sensitivity was reduced.
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