Comparison of four molecular markers in measuring relationships among the wild potato relatives Solanum section Etuberosm (Subgenus Potatoe)
1996
Spooner, D.M. | Tivang, J. | Nienhuis, J. | Miller, J.T. | Douches, D.S. | Contreras-M, A.
We evaluated chloroplast DNA (cpDNA), isozymes, single to low-copy nuclear DNA (RFLPs), and random amplified polymorphic DNAs (RAPDs) in terms of concordance for genetic distance of 15 accessions each of Solanum etuberosum and S. palustre, and 4 accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Etuberosum, a group of nontuber-bearing species closely related to Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimensional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs.
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