Sequence of the domestic duck (Anas platyrhynchos) growth hormone-encoding gene and genetic variation in the promoter region
Kansaku, Norio | Soma, Aya | Furukawa, Satoko | Hiyama, Gen | Okabayashii, Hisato | Guemene, Daniel | Kuhnlein, Urs | Zadworny, David
The duck growth hormone encoding gene and its promoter region were amplified by polymerase chain reaction (PCR). A total of 5.25 kb were cloned and sequenced. Duck growth hormone (GH) consists of five exons and four introns and is structurally similar to mammalian and chicken GH gene. Although the distal region of duck GH promoter showed no similarity to chicken and turkey promoters, the proximal region of the promoter contained two putative Pit-1 binding sequences, and showed similarity to chicken and turkey GH promoters. Genetic variation was detected at five positions of the promoter region. The results of this study indicate that the expression of duck GH is likely regulated in a similar manner to that of chicken GH via enhancer-type cis-acting elements and the presence of genetic variation in the duck GH gene may be applicable to marker-assisted selection.
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