Synthesis of propyl gallate by mycelium-bound tannase from Aspergillus niger in organic solvent
2007
Yu, Xiao-Wei | Li, Yong-Quan | Zhou, Shi-Miao | Zheng, Yu-Yi
Aspergillus niger with mycelium-bound tannase activity was employed to investigate the synthesis of propyl gallate from gallic acid and 1-propanol in organic solvents. The effects of various organic solvents (log P: -1.0 to 6.6) on the enzymatic reactions showed that benzene (log P: 2.0) was the most suitable solvent. The water content and protonation state of mycelium-bound enzyme both had significant effects on the activity of tannase. The maximum molar conversion (65%) was achieved with 7.3% (v/v) 1-propanol and 5.56 mM gallic acid at stirring speeds of 200 rev/min, 40 °C in presence of anhydrous sodium sulfate and PEG-10,000. Enzyme specificity for the alcohol portion (C₁-C₈) of the ester showed that the optimum synthesis was observed with alcohols ranging from C₃ to C₅.
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