Variation in the content of hypericins in four generations of seed progeny of Hypericum perforatum somaclones
Koperdáková, Jana | Košuth, Ján | Čellárová, Eva
The content of hypericins in in vitro regenerated Hypericum perforatum plants (R ₀) and four generations of their seed progeny (R ₁–R ₄) was compared. The mean content of hypericins in field-grown plants over the period 1992–2002 gradually increased under selection, and in the R ₄ generation it was almost seven-times higher than that in the R ₀ somaclones. Significant difference between hypericin content in diploids and tetraploids was detected in R ₀, R ₁ and R ₃ generations. Hypericin content in four diploid and tetraploid lineages originated from a single somaclone was genotype dependent. To eliminate the influence of environmental conditions during different growing seasons, we used seeds of selected R ₀–R ₃ plants to derive R′₁ to R′₄ generations cultivated during the same years. In this case no statistically significant difference in hypericin content was found between the R′₁–R′₄ generations. Apomictically and sexually derived plants were distinguished by PCR using variable numbers of tandem repeats (VNTR) primers. The content of hypericins in apomictically derived progenies was compared.
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