Determination of water-insoluble β-D-glucan in the whole-grain cereals and pseudocereals
Hozová, B. | Kuniak, Ľ | Moravčíková, P. | Gajdošová, A.
Water-insoluble β-(1,3)-D-glucan (lichenan) was determined in 43 samples of various cereal (oats, barley, wheat, millet) and pseudocereal (buckwheat, amaranth) cultivars using a modified procedure with fungal α-amylase (Fermizyme P 300). The content of water-insoluble β-glucan varied in the dependence on the cereal species and cultivars. The highest content was registered in covered oat cultivars (Cyril and the new breeding cultivar PS-100) ranging from 26.7 to 28.2 g/100 g dry matter (d.m.) followed by less traditional cereals such as millet (<I>Panicum miliaceum</I> L.), amaranth <I>(Amaranthus</I> sp. L.), and buckwheat (<I>Fagopyrum</I>) – more than 20 g/100 g d.m. A somewhat lower average content of water-insoluble β-glucan was found in wheat – 12.7–16.2 g/100 g d.m., in spelt wheat – 8.5 g/100 g d.m., and in oats – varying between 15.3 and 18.7 g/100 g d.m.
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