Molecular structural and functional characterization of STAT1 gene regulatory region in teleost Channa argus
2010
Jia, Weizhang | Zhou, Xiuxia
The transcription factor STAT1 is involved in signal transduction of type I and II interferons (IFNs). However, the molecular characteristics of the STAT1 regulatory region still remain to be elucidated in teleosts. In the present study, the complete cDNA and the regulatory region of the STAT1 gene were isolated from snakehead (Channa argus). More than 2.4kb 5′-flanking region of STAT1 shares the regulatory elements of IFN-stimulated response element (ISRE) and IFN-γ activation site (GAS). Consensus ISRE and GAS were located from −373 to −361 and −716 to −724 in the promoter region, respectively. Moreover, it is noticeable that the crucial elements of ISRE (+698 to +710) and GAS (+294 and +301) are present in the first intron of snakehead STAT1. Comparisons of six vertebrate STAT1 5′-flanking regions all present the common sequence characteristics of IFN-induced gene promoter, which include ISRE, GAS and Sp1 sites. In order to further characterize the snakehead STAT1 regulatory region, six reporter constructs of snakehead STAT1 promoter and first intron were generated to examine the specificity to human interferon-gamma (hIFN-γ). Only those constructs containing the ISRE element showed notable reporter activity after stimulation of Hela cells with hIFN-γ. However, sequential deletions of putative transcription factor binding sites indicated that GAS elements have little effect on the promoter and intronic activity in response to hIFN-γ. Taken together, these results suggest that the regulatory mechanisms of IFN-signalling appear to be mediated in a similar manner in fish and mammals.
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