Morphological deformation of Daphnia magna embryos caused by prolonged exposure to ibuprofen

Grzesiuk, Malgorzata; Pijanowska, Joanna; Markowska, Magdalena; Bednarska, Anna

Morphological deformation of Daphnia magna embryos caused by prolonged exposure to ibuprofen

2020

Grzesiuk, Malgorzata | Pijanowska, Joanna | Markowska, Magdalena | Bednarska, Anna

The effect of chronic exposure of freshwater cladoceran Daphnia magna to low, environmentally relevant concentrations i.e 4 μgL⁻¹of ibuprofen (a nonsteroidal anti-inflammatory drug) in a laboratory experiment was studied. We observed the key life history traits of first and fifth generation individuals: age and size at first reproduction, number of first clutch eggs and individual growth rate. Moreover, chosen molecular/subcellular markers of experimental animals stress response such as triglyceride content, heat shock proteins (HSP) expression and DNA:RNA ratio were collected. Overall, chronic exposure to ibuprofen had no significant effect on the molecular markers nor on the life history parameters of the Daphnia. It did, however, caused lethal morphological deformities in embryos and juvenile daphnids. Depending on the clonal affiliation, exposure to a low dosage of ibuprofen over five generations resulted in the deformation of ∼3%–∼10% of the first clutch of offspring. Also, up to 90% of females carried at least one deformed embryo. This is the first time that research has revealed such an effect of ibuprofen on D. magna.

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