Real-time monitoring of T-cell-secreted interferon-γ for the diagnosis of tuberculosis
Wu, Changlin | He, Jianan | Li, Boan | Xu, YunQing | Gu, Dayong | Liu, Houming | Zhao, Dan | Shao, Chaopeng
In this paper, we report the development of a label-free biosensor, based on surface plasmon resonance, for real-time monitoring of captured human CD4⁺T-cells, and their dynamic cytokine production upon specific antigen stimulation. Microarrays of CD4⁺T-cells, and interferon gamma (IFN-γ) specific antibody (Ab) spots were printed side by side onto the poly(OEGMA-co-HEMA) matrix. The placement of CD4⁺ T-cells near the anti IFN-γ Ab-coated spots ensured a high local concentration of secreted IFN-γ for detection. We have demonstrated that this approach enables the detection of tuberculosis (TB) infection in clinical samples, with an overall sensitivity of 85.5% and an overall specificity of 97.7%.
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