Ochrobactrum pecoris sp. nov., isolated from farm animals
Kämpfer, Peter | Huber, Bettina | Busse, Hans-Jürgen | Scholz, Holger C. | Tomaso, Herbert | Hotzel, Helmut | Melzer, Falk
Two Gram-negative, rod-shaped, non-spore-forming strains, designated 08RB2639T and 08RB2781-1, were isolated from a sheep (Ovis aries) and a domestic boar (Sus scrofa domestica), respectively. By 16S rRNA gene sequencing, the isolates revealed identical sequences and were shown to belong to the ALPHAPROTEOBACTERIA: They exhibited 97.8 % 16S rRNA gene sequence similarity with Ochrobactrum rhizosphaerae PR17T, O. pituitosum CCUG 50899T, O. tritici SCII24T and O. haematophilum CCUG 38531T and 97.4 % sequence similarity with O. cytisi ESC1T, O. anthropi LMG 3331T and O. lupini LUP21T. The recA gene sequences of the two isolates showed only minor differences (99.5 % recA sequence similarity), and strain 08RB2639T exhibited the highest recA sequence similarity with Ochrobactrum intermedium CCUG 24694T (91.3 %). The quinone system was ubiquinone Q-10, with minor amounts of Q-9 and Q-11, the major polyamines were spermidine, putrescine and sym-homospermidine and the major lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine, with moderate amounts of the Ochrobactrum-specific unidentified aminolipid AL2. The major fatty acids (>20 %) were C18 : 1Ï7c and C19 : 0 cyclo Ï8c. These traits were in excellent agreement with the assignment of the isolates to the genus OCHROBACTRUM: DNA–DNA relatedness and physiological and biochemical tests allowed genotypic and phenotypic differentiation from other members of the genus OCHROBACTRUM: Hence, it is concluded that the isolates represent a novel species, for which the name Ochrobactrum pecoris sp. nov. is proposed (type strain 08RB2639T = DSM 23868T = CCUG 60088T = CCM 7822T).
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