Reduced water activity during sporogenesis in selected penicillia: impact on spore quality
1998
Blaszyk, M. | Blank, G. | Holley, R. | Chong, J.
Heat resistance germination and sensitivity to selected antimicrobials was investigated using Penicillium roqueforti, Penicillium aurantiogriseum and Penicillium viridicatum spores, produced on potato dextrose agar (PDA) adjusted to 0.88 aw using either glycerol or NaCl. Results indicated that spores produced on media without aw adjustment (0.99 aw; control) appeared more heat resistant (55 degrees C, 10 min) compared to those produced at 0.88 aw (treatment). For treatment spores only, heat resistance was observed to decrease further when initially incubated (25 degrees C, 48 h) in sterile, distilled water. All treatment spores were more sensitive to benzoate and sorbate but more resistant to cycloheximide. The time taken for germination, especially for P. roqueforti spores appeared to vary depending on the aw and solute used during spore production.
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