Predicting the number of dominant R alleles in single wheat kernels using visible and near-infrared reflectance spectra
1999
Wang, D. | Dowell, F.E. | Lacey, R.E.
An optical radiation measurement system was used to measure reflectance spectra of single wheat kernels from 400 to 2,000 nm. A total of 18 wheat samples with 0, 1, 2, or 3 R alleles for red grain color was used for this study. The results indicated a linear relationship between the degree of the red pigmentation and the number of R alleles. The highest coefficient of determination (R2) was 0.78 in the wavelength region of 500-1,700 nm when a four-class partial least squares model was used. The highest classification of red genes was 78.4%. For two-class models, differentiating samples with 0 R alleles and 1 R allele from samples with 3 R alleles had the highest success rate of 100 and 98.8%, respectively. The number and combination of R alleles had a significant effect on wheat kernel color. These relationships may be useful to wheat breeders in estimating the number and location of R alleles.
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