Flavanol production by Fagopyrum esculentum hairy and normal root cultures
Trotin, F. | Moumou, Y. | Vasseur, J.
Hairy roots culture of Fagopyrum esculentum were established by infection with Agrobacterium rhizogenes strain 15834. Faster growth (four-fold higher than normal roots culture) in B5 liquid medium was observed and the synthesis of five flavanols obtained:(+)-catechin, (-)-epicatechin, (-)-epicatechin-3-O-gallate, procyanidin B2 and procyanidin B2-3'-O-gallate also present in normal roots culture with a common prominency of epicatechin-3-O-gallate synthesis and inversion in the contents rates of (+)-catechin and (-)-epicatechin. The highest content of procyanidin B2-3'-O-gallate was obtained in hairy roots which can be considered as a better production source for flavanols, especially of the two galloylated derivatives.
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