beta-D-Glucan antibodies inhibit auxin-induced cell elongation and changes in the cell wall of Zea coleoptile segments
1989
Hoson, T. | Nevins, D.J.
Antiserum was raised against the Avena sativa L. caryopsis beta-glucan fraction with an average molecular weight of 1.5 X 10(4). Polyclonal antibodies recovered from the serum after Protein A-Sepharose column chromatography precipitated when cross-reacted with high molecular weight (1 leads to 3), 1 leads to 4)-beta-D-glucans. These antibodies were effective in suppression of cell wall autohydrolytic reactions and auxin-induced decreases in noncellulosic glucose content of the cell wall of maize (Zea mays L.) coleoptiles. The results indicate antibody-mediated interference with in situ beta-D-glucan degradation. The antibodies at a concentration of 200 micrograms per milliliter also suppress auxininduced by the minimum stress-relaxation time of the segments) of Zea coleoptiles. The suppression of elongation by antibodies was imposed without a lag period. Auxin-induced elongation, cell wall loosening, and chemical changes in the cell walls were near the levels of control tissues when segments were subjected to antibody preparation precipitated by a pretreatment with Avena caryopsis beta-D-glucans. These results support the idea that the degradation of (1 leads to 3), (1 leads to 4)-beta-D-glucans by cell wall enzymes is associated with the cell wall loosening responsible for auxininduced elongation.
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