Ovine pulmonary adenocarcinoma: Evaluation of molecular tumour markers
2007
Pawaiya, R.V.S. | Kumar, Ram
Ovine pulmonary carcinoma (previously pulmonary adenomatosis) is characterized by Jaagsiekte sheep retrovirus-induced progressive alveolar epithelial proliferation, resulting into papillary ingrowths and associated fibroplasia of alveolar septa. The diagnostic efficacy of certain molecular tumour markers was evaluated by immunohistochemistry on formalin-fixed paraffin-embedded sections using specific monoclonal antibodies. Out of total nine cases, one was found to be metastasized in the mediastinal lymph node and liver. Histopathologically, papilliform alveolar epithelial cells assuming large cuboidal to columnar appearance, obliterating partially or completely the alveolar spaces, associated fibrosis of alveolar septa and infiltration of inflammatory cells were seen. AgNOR staining revealed many small AgNOR dots (mean value 11.64±0.94 per nucleus) and mean PCNA index was 73.90±29.39, suggesting proliferative activity of the alveolar epithelial cells. The cells were positive for p53, c-Myc, PCNA and hTERT, showing specific nuclear staining. The proliferating cells showed reduced expression of cadherin. Strong fibronectin presence was seen in the stroma and connective tissue while proliferating alveolar epithelial cells revealed relatively weak pericellular fibronectin expression. The results showed the successful use of the tumour markers in characterizing the neoplastic properties of the alveolar epithelial cells in the ovine pulmonary adenocarcinoma.
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