Somatic embryogenesis and analysis of peroxidase in cultured lettuce (Lactuca sativa L.) cotyledons

Zhou, X.L.; Han, Y.; Yang, W.J.; Xi, T.

Somatic embryogenesis and analysis of peroxidase in cultured lettuce (Lactuca sativa L.) cotyledons

1992

Zhou, X.L. | Han, Y. | Yang, W.J. | Xi, T.

Somatic embryos were induced in lettuce cotyledons cultured on Murashige and Skoog's (MS) medium containing either 2 mg 1-1 6-benzylaminopurine (BA) and 0.2 mg 1-1 naphthaleneacetic acid (NAA) or 0.2 mg 1-1 BA and 2 mg 1-1 NAA. Both combinations induced a frequency of over 70%. The explants cultured only in the presence of 2,4-dichlorphenoxyacetic acid (2,4-D) did not produce somatic embryos. The development of the embryoids was studied histologically and by scanning electron microscopy. Peroxidase activity was assayed and the isoenzyme pattern of calluses was determined by polyacrylamide gel electrophoresis. Callus from an embryogenic line showed a much higher peroxidase activity than that from a non-embryogenic line, one extra peroxidase isozyme band being present and typical of the embryogenic callus. No qualitative differences were detectable between the embryogenic calluses.

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