Pulsed electric field for increasing intracellular trehalose content in Lactobacillus plantarum WCFS1
Vaessen, E.M.J. | den Besten, H.M.W. | Patra, T. | van Mossevelde, N.T.M. | Boom, R.M. | Schutyser, M.A.I.
Pulsed electric field (PEF) processing has been developed and applied in food industry for several purposes. In this study we used PEF for increasing the intracellular trehalose content in Lactobacillus plantarum WCFS1. Our results indicated that it is possible to increase intracellular trehalose content in Lactobacillus plantarum WCFS1 to ~100 mM with 75% survival when applying a PEF treatment with an electric field strength of 7.5 kV/cm. Fluorescence staining of PEF-treated cells with propidium iodide (PI) and SYTO 9 showed that at 7.5 kV/cm only a small fraction (23%) of the cells had a permeated membrane by this PEF treatment, of which approximately half had an irreversible permeated membrane. Resealing of the pores in the membrane for PI uptake was very fast, in the order of seconds. These results indicate that PEF treatment is promising for increasing intracellular trehalose, but further optimization is required to increase the trehalose content in all cells.The market for probiotics is growing. Probiotic survival during processing steps such as spray drying is essential for their beneficial effect. We studied pulsed electric field treatment as a method to increase the intracellular trehalose content in L. plantarum WCFS1 which could enhance bacterial robustness during processing. This increased bacterial robustness may again contribute to more energy efficient processing routes of probiotic foods.
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