First detection of free d-amino acids in human nails by combination of derivatization and UPLC-ESI-TOF-MS
2010
Min, Jun Zhe | Hatanaka, Suguru | Yu, Hai-fu | Higashi, Tatsuya | Inagaki, Shinsuke | Toyo'oka, Toshimasa
The resolution of free dl-amino acids in the human nail was carried out by combination of the R(−)-DBD-PyNCS derivatives and UPLC-ESI-TOF-MS. As we know, for the first time, these five kinds of d-amino acids, were determined from human nail samples. Fifteen kinds of l-amino acids were also recognized from human nails. Application to dl-amino acids in healthy person and diabetes patients nails is also described in this communication.
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