Metabolism mediated interaction of α-asarone and Acorus calamus with CYP3A4 and CYP2D6
2011
Pandit, Subrata | Mukherjee, Pulok K. | Ponnusankar, Sivasankaran | Venkatesh, Murugan | Srikanth, N.
The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC₅₀ values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC₅₀ values (46.84±1.83–32.99±2.21μg/ml) comparing to the standard inhibitors and lower IC₅₀ value than α-asarone (65.16±2.37–42.15±2.45μg/ml).
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