Assessment of genetic and phenotypic variation among intraspecific somatic hybrids of potato, Solanum tuberosum L
1999
Gavrilenko, T. | Thieme, R. | Tiemann, H.
Intraspecific somatic hybrids have been produced by protoplast fusion in eight combinations involving 10 dihaploids (2n = 2x = 24) in an attempt to provide new material for potato breeding. Cytological analysis revealed extensive variation in chromosome number, such as aneuploid, aneusomatic and mixoploid hybrids. Most of the hybrids represented the expected chromosome number of 48; however, the frequency of aneuploids reached 50% in some combinations. Some hybrids carried structurally rearranged chromosomes and exhibited a high frequency of aberrant anaphases. Isozyme and random amplified polymorphic DNA (RAPD) patterns of the hybrids from the same fusion combination were uniform. In the field, somatic hybrids showed wide phenotypic variation in 20 morphological characters. There was a significant correlation between certain leaf characters and the ploidy level, which may be used to distinguish the tetraploid hybrids from hexaploids and octoploids. Tuber yield and flowering intensity were highest in tetraploid hybrids (2n = 4x = 48). Eighteen of the 73 hybrids reached higher yields than the standard variety 'Adretta'. Floral development and fertility were restored in hybrids derived from fusions between non-flowering or sterile dihaploids.
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