An electrochemiluminescence biosensor for cadmium ion based on target-induced strand displacement amplification and magnetic Fe3O4-GO nanosheets
2022
Xu, Huifeng | Zhang, Shiqi | Zhang, Ting | Huang, Weihua | Dai, Yuting | Zheng, Ruoxi | Wu, Guangwen
Taking advantage of an exquisite hairpin DNA for strand displacement amplification (SDA) and the magnetic Fe₃O₄-graphene oxide nanosheets (MGN) as the carrier, an immobilization-free ECL biosensor was constructed for ultra-trace detection of Cd²⁺. Firstly, the ECL probe Ru (phen)₃²⁺ easily diffuses in the solution and reaches the electrode surface to induce strong ECL signal. This is because the pre-designed hairpin DNA is constrained by MGN in the absence of Cd²⁺. The presence of Cd²⁺ releases cDNA by binding to its corresponding aptamer, leading to removal of hairpin DNA away from the surface of MGN. In this case, SDA amplification was evoked and generated numerous dsDNA which further trapped Ru (phen)₃²⁺ in its groove. It is difficult for the embedded ECL probe to touch the electrode surface to generate ECL signal. Therefore, the concentration of Cd²⁺ was monitored according to the attenuation of ECL signal. This method showed high sensitivity to Cd²⁺ with a detection limit of 1.1 × 10⁻⁴ ppb. Moreover, it not only avoids many condition optimizations required in the conventional SDA method, but also circumvent the modification and immobilization of DNA probe. This sensor is further applied in the detection of Cd²⁺ in the sample of traditional Chinese medicine.
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Эту запись предоставил National Agricultural Library