Post-mortem activity of the glycogen debranching enzyme and change in the glycogen pools in porcine M. longissimus dorsi from carriers and non-carriers of the RN-gene
Yla-Ajos, M.S.K. | Lindahl, G. | Young, J.F. | Theil, P.K. | Puolanne, E. | Enfalt, A.C. | Andersen, H.J. | Oksbjerg, N.
Glycogen debranching enzyme (GDE) is together with glycogen phosphorylase responsible for the degradation of glycogen. The present study compares the post-mortem activity of GDE and breakdown of the glycogen pools in M. longissimus dorsi of RN- carrier pigs and in wild type animals. The activity of GDE (n = 14) and pH (n = 20) was measured 0.5, 3, 5, 24 and 48 h post-mortem. The change in pro-glycogen and in macro-glycogen content (n = 20) was followed until 216 h post-mortem and the transcription level of GDE, glycogenin and glycogen synthase m-RNA (n = 19) were measured 0.5 h post-mortem. Both the activity of GDE and the transcription level of GDE were found to be similar in RN- carriers and wild type animals shortly after slaughter. However, the activity declined faster in wild type animals compared with RN- carriers with increasing time post-mortem. The contents of both pro-glycogen and macro-glycogen were higher in RN- carriers compared with wild type animals, and further, the proportion of macro-glycogen was higher in RN- carriers compared with wild type animals. During the post-mortem period, only degradation of pro-glycogen was observed in both genotypes. The decrease in pH was faster and the ultimate pH lower in RN- carriers than in wild type animals. It was suggested that the higher GDE activity in the late phase of the post-mortem period in muscles from RN- carriers renders the extended pH decrease in these muscles.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library