Application of random amplified polymorphic DNA markers to evaluate intraspecific genetic variation in the Elymus alaskanus complex (Poaceae)
2002
Zhang, X.Q. | Salomon, B. | Bothmer, R. von
Random amplified polymorphic DNA (RAPD) markers were used to evaluate the levels and patterns of genetic diversity in ten Elymus alaskanus populations, which were collected from Canada, USA, Greenland and Russia. Ten arbitrarily chosen decamer primers were used in this study. The results revealed high levels of variation. The mean number of alleles per locus (Ap) was 1.5, ranging from 1.4 to 1.6, the mean percent of polymorphic loci (Pp) was 49.5%, ranging from 35.1% to 64.9%, and the mean gene diversity (Hep) was 0.162, varying from 0.142 to 0.262. The total variation was H(T) = 0.403. When partitioned (G(ST)), 60% of the total variation was found among the populations. Although the genetic diversity values obtained with RAPDs are much higher than for allozymes, they are similar regarding how the genetic variation is distributed among populations. In addition, a similar genetic pattern of population differentiation, where populations from Greenland and the USA ("violaceus" and "latiglumis") were clearly separated from the others ("hyperarcticus", "komarovii" and "sajanensis"), was revealed by both the cluster and principal coordinates analyses.
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