Regulation of alpha-amylase isoenzyme expression in Araucaria araucana by gibberellic and abscisic acids
1997
Acevedo, E. | Cardemil, L.
Expression of alpha-amylase (EC 3.2.1.1) isoenzymes present in the embryos and mega-gametophytes of Araucaria araucana seedlings was studied under gibberellic acid (GA3) and abscisic acid (ABA) treatments and after 90 hr of imbibition in 20 mM of beta-chloroethyltrimethylammonium (CCC), a GA3 biosynthesis inhibitor. When CCC was used, alpha-amylase activity in the treated embryos decreased to 28% with respect to the controls. The isoenzymes of Rm 0.42 and 0.51 were greatly affected. When the embryos were imbibed simultaneously in the presence of 20 mM CCC and 10(-5) M of GA3, alpha-amylase activity and the expression of isoenzymes were restored with respect to controls. ABA at 10(-4) and 10(-5) M decreased alpha-amylase activity of embryos to 67 and 81%, respectively. Expression of isoenzymes of Rm 0.51 was greatly decreased by ABA but the effect on the others was small. Isoenzyme expression of mega-gametophytic alpha-amylase was not affected by CCC.
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