Shoot organogenesis from cotyledons of sunflower / organogenesis de la tallos cotiledones de girasol / organogenese a partir des cotyledons de tournesol
2014
Azadi, P. | Moieni, A. | Ahmadi, M.R.
The experiment was conducted in order to study the regeneration potential of cotyledon explants from some Iranian inbred lines of sunflower (Helianthusannuus L.) and their F₁ hybrids. Cotyledons of 2-day-old seedlings of three cytoplasmic male sterile inbred lines used as female (CMS 24, CMS 31 and CMS 60/52) and five inbred lines used as male (R-81.1, R-92, R-97, R-201 and R-232) and finally their 15 F₁ hybrids were plated on shoot induction medium, Murashige and Skoog (MS) containing 3% sucrose, 0.5% agar-agar and different concentrations of BAP and NAA. Analysis of variance showed significant differences for all organogenesis traits studied among parental genotypes and F₁ hybrids, hormone combination treatments and genotype × hormone combination treatment interaction. Shoot organogenesis was optimized on MS medium with 1 mg l⁻¹ BAP and 1 mg l⁻¹ NAA.Interaction “(CMS 24 × R-201) × T2” showed the highest values for number of explants shooting per 100 explants plated (55%). Interaction “R- 92× T2” showed the highest value for average number of shoots per explants plated (8.65). Interaction “R-81.1 × T2” showed the highest value for average number of shoots per explants shooting (23). Interaction “CMS 24 × T2” showed the highest value for number of explants rooting per 100 explants plated (35%).
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