Phytochemical screening and evaluation of antioxidant and antibacterial activities of Origanum compactum extracts | Screening phytochimique et évaluation de l’activité antioxydante et antibactérienne des extraits d’Origanum compactum
2017
Bouyahya, A. | Abrini, J. | Bakri, Y. | Dakka, N.
Antibacterial and antioxidant activities of organic extracts of oregano (Origanum compactum Benth.) were evaluated. The antibacterial activity was evaluated by well diffusion method. The antioxidant activity was estimated by radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The total phenol content and flavonoids were determined by the Folin–Ciocalteu method and AlCl₃ method, respectively. All samples were proved to be capable of inhibiting bacterial strains tested with the largest zone of inhibition of about 34 ± 1.24 mm. The Gram negative bacteria are more resistant than Gram positive extracts. The n-hexane extract showed the strongest antibacterial activity, while the ethyl acetate extract showed the lowest antibacterial activity.The quantitative evaluation of the anti-radical activity has shown that n-hexane extract is most active (IC₅₀ = 39.83 μg/ml). However, ascorbic acid (used as a control) showed an activity about equivalent (IC₅₀ = 27.20 mg/ml), indicating the presence of effective compounds in the biochemical composition of the plant that have a high capacity in the reduction of DPPH. Quantification of total polyphenols and flavonoids showed higher values with MeOH extract, where the highest value was estimated by a spectrophotometer: 153.27 ± 0.68 mg GAE (Gallic Acid Equivalent)/ g of extract; 52.72 ± 0.72 mg QE (Qercetin Equivalent)/ g of extract.
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