Estimation of minimal numbers of Azospirillum brasilense using time-limited liquid enrichment combined with enzyme-linked immunosorbent assay
1991
Bashan, Y. | Mitiku, G. | Ziv-Vecht, O. | Levanony, H.
A simple time-limited liquid enrichment procedure to aid in the quantitative detection of very few cells of Azospirillum brasilense in plant roots is described. The method is based on limited multiplication of A. brasilense in convential semi-solid medium and counting of the bacteria in the enriched medium by enzyme-linked immunosorbent assay (ELISA) or by Most Probable Number (MPN) techniques. The method can be used as a complementary procedure to ELISA and MPN techniques when low numbers of A. brasilense are present in the roots.
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