ASu@MNPs-based electrochemical immunosensor for vitamin D3 serum samples analysis
2023
Polli, Francesca | D'Agostino, Cristine | Zumpano, Rosaceleste | De Martino, Viviana | Favero, Gabriele | Colangelo, Luciano | Minisola, Salvatore | Mazzei, Franco
We report a new sensitive label-free electrochemical immunosensor to detect Vitamin D₃ (25-OHD₃) in untreated serum samples. To this aim, a graphite screen printed electrode (SPE) was modified using cysteamine (CYM) functionalized core-shell magnetic nanoparticles (Au@MNPs) then, the 25-OHD₃ antibody (AbD) was immobilized via glutaraldehyde crosslinking. The several steps involved in the immunosensor development and 25-OHD₃ analysis were monitored by using differential pulse voltammetry (DPV). The developed immunosensor showed a LOD of 2.4 ng mL⁻¹ and a linear range between 7.4 and 70 ng mL⁻¹. The effectiveness of the immunosensor in human serum analysis was assessed by comparing the results obtained with the chemiluminescence-immunoassay (CLIA) reference method. The high sensitivity and excellent agreement with the reference method suggest its potential use as a POCT to monitor hypovitaminosis 25-OHD levels.
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