Inhibition of Bacillus licheniformis spore growth in milk by nisin, monolaurin, and pH combinations
1999
Mansour, M. | Amri, D. | Bouttefroy, A. | Linder, M. | Milliere, J.B.
The effects of nisin and monolaurin, alone and in combination were investigated on Bacillus licheniformis spores in milk at 37 degrees C. In the absence of inhibitors, germinated spores developed into growing vegetative cells and started sporulation at the end of the exponential phase. In the presence of nisin (25 IU ml-1), spore outgrowth was inhibited (4 log10 reduction at 10 h). Regrowth appeared between 10 and 24 h and reached a high population level (1(.)25 X 10(8) cfu ml-1) after 7d. Monolaurin (250 microgram ml-1) had a bacteriostatic effect during the first 10 h but thereafter, regrowth occurred slowly with a population level after 7 d (4 X 10(5) cfu ml-1) lower than that of nisin. Different combined effects of nisin (between 0 and 42 IU ml-1), monolaurin (ranging from 0 to 300 microgram ml-1), pH values (between 5(.)0 and 7(.)0) and spore loads (10(3), 10(4), 10(5) spores ml-1) were investigated using a Doehlert matrix in order to study the main effects of these factors and the different interactions. Results were analysed using the Response Surface Methodology (RSM) and indicated that nisin and monolaurin had no action on spores before germination; only pH values had a significant effect (P less than equal to 0(.)001), i.e. spore count decreased as the pH value increased in relation to germination. Sublethal concentrations of nisin (30 IU ml-1) and monolaurin (100 microgram ml-1) in combination acted synergistically on outgrown spores and vegetative cells, showing total inhibition at pH 6(.)0, without regrowth, within 7 d at 37 degrees C.
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