Heterologous expression of the lndYR and wblA gₕ genes in Streptomyces nogalater LV65, S. echinatus DSM40730, and S. peucetius subsp. Caesius ATCC27952 (producers of anthracycline antibiotics)
2013
Klymyshin, D. O. | Nimets, O. Ya | Stefanyshyn, O. M. | Fedorenko, V. O.
Strains of the actinomycetes Streptomyces nogalater Lv65, S. echinatus DSM40730, and S. peucetius subsp. caesius ATCC27952 are producers of the anthracycline antibiotics nogalamycin, aranciamycin, and doxorubicin, respectively. This work was focused on the impact produced by the expression of the regulatory lndYR and wblA gₕ genes on the secondary metabolism and morphogenesis of these bacteria. Introducing regulators into the composition of replicative plasmids in the streptomycetes in question leads to a decrease in the synthesis of anthracyclines, whereas the expression of lndYR in cells of S. peucetius subsp. caesius ATCC27952 suppresses the sporulation of the doxorubicin producer which may indicate the presence of their homologues in the genomes. The identification of these genes for the purpose of their further directed inactivation may be a successful tool for obtaining strains with an increased level of synthesis of clinically important compounds, as well as it can allow us to establish particular stages in the regulation of the secondary metabolism of anthracycline antibiotics.
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