Enhanced surfactin fermentation via advanced repeated fed-batch fermentation with increased cell density stimulated by EDTA–Fe (II)
2021
Mei, Yuwei | Yang, Ziyun | Kang, Zhihao | Yu, Fan | Long, Xuwei
High-performance production of biosurfactant surfactin is very important for real applications of surfactin but still exhibits significant challenges. In this study, an efficient fermentation process with enhanced cell density is developed to improve the surfactin production by Bacillus subtilis ATCC 21332. The addition of 0.2 mM EDTA–Fe²⁺ complex resulted in the highest surfactin yield of 5.67 g/L within 3 days, which is 7.8 times higher than that obtained by regular fermentation in the absence of this complex. This significantly improved surfactin production is related to the remarkably increased cell density which was stimulated by the addition of EDTA-Fe²⁺ complex at molar ratio of 1:1, was significantly better than that obtained by the addition of Fe²⁺, Fe³⁺ or EDTA alone. Furthermore, a modified repeated fed-batch fermentation process sustained the high-performance fermentation of surfactin at 1.345 g/L d, and the overall surfactin yield reached 9.414 g/L within a fermentation period of 7 days.
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