The starch-binding domain as a tool for recombinant protein purification [Erratum: October 2021, Vol.105(19), p.7551-7552]
2013
Guillén, D. | Moreno-Mendieta, S. | Aguilera, P. | Sánchez, S. | Farres, A. | Rodríguez-Sanoja, R.
Recombinant protein purification with affinity tags is a widely employed technique. One of the most common tags used for protein purification is the histidine tag (Hisₜₐg). In this work, we use a tandem starch-binding domain (SBDₜₐg) as a tag for protein purification. Four proteins from different sources were fused to the SBDₜₐg, and the resulting fusion proteins were purified by affinity chromatography using the Hisₜₐg or the SBDₜₐg. The results showed that the SBDₜₐg is superior to the Hisₜₐg for protein purification. The efficient adsorption of the fusion proteins to raw corn starch was also demonstrated, and two fusions were selected to test purification directly using raw starch from rice, corn, potato, and barley. The two fusion proteins were successfully recovered from crude bacterial extract using raw starch, thus demonstrating that the SBDₜₐg can be used as an efficient affinity tag for recombinant protein purification on an inexpensive matrix.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил National Agricultural Library