The influence of auxin and cytokinin on proliferation and morphology of somatic embryos of Picea abies (L.) Karst
1992
Bellarosa, R. | Mo, L.H. | Arnold, S. von
Embryogenic cell-lines of Picea abies were initiated from mature zygotic embryos and cultured on medium containing 2,4-D and BA. The cell-lines were categorized into two main groups (solar/polar and undeveloped embryos), based on the morphology of the somatic embryos and their ability to go through a maturation process when treated with ABA. The cell-lines were transferred to media containing (1) 2,4-D and BA; (2) only BA: (3) only 2,4-D or (4) no growth regulators. When cultured on a medium containing both 2,4-D and BA new somatic embryos were continually formed. In contrast, when they were cultured without one or both of the growth regulators no new somatic embryos were formed. Solar/polar and undeveloped embryos responded in the same way. On a medium containing only BA the somatic embryos already present increased in size and developed an extremely large embryonic region. On a medium containing only 2,4-D the embryos already present became disorganized into loose aggregates. When transferred from a medium containing both 2,4-D and BA to one containing ABA, mature somatic embryos developed from the solar/polar type but not from the undeveloped type. The ability of the solar/polar somatic embryos to go through a maturation process decreased when they were precultured on a medium lacking auxin and was lost when they were precultured on medium lacking cytokinin. The cell-lines containing undeveloped somatic embryos produced mature somatic embryos in one cell-line out of three that had been cultured on the medium containing only BA.
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