Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on the chromosome-1 of B. patellaris and B. procumbens
1994
Salentijn, E.M.J. | Sandal, N.N. | Klein-Lankhorst, R. | Lange, W. | Bock, T.S.M. de | Marcker, K.A. | Stiekema, W.J.
New members of a satellite DNA family (Sat121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1)in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1Pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat121 clusters were detected near the Hs1pro-1 locus.
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