Amanitin Content and Toxicity of Amanita verna Bull
2014
Seeger, Ruth | Stijve, Tjakko
The α-, β-, and γ-amanitin content of 11 samples of Amanita verna Bull., collected during 1975 - 1978 in Germany and Switzerland, has been determined by high performance thin-layer chromatography (HPTLC) of crude methanolic extracts. The toxicity (i. v. LD₅₀ for mice of defatted, lyophilized, methanolic extracts) of 3 samples has been compared with that of A.phalloides from the same site of collection.The amanitin content of A. verna ranged from 2250 to 4570 mg/kg dry weight; the fungi contained almost as much β- as α-amanitin, whereas the γ-amanitin content amounted to about 12% of the total amanitin. A. verna contained less amanitin (65% on the average) than A. phalloides from the same collection site, but it was not significantly less toxic, since the phallotoxins contributed to the toxicity of either species in our tests.
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