Establishment of a continuous cell line from southern bluefin tuna (Thunnus maccoyii)
2013
Bain, Peter A. | Hutchinson, Rhonda G. | Marks, Alexandra B. | Crane, Mark St.J. | Schuller, Kathryn A.
A continuous cell line, designated SBT-E1, was established from tissues of a southern bluefin tuna (Thunnus maccoyii) fingerling. Maximum cell proliferation rates occurred at 25°C in Liebovitz's L-15 medium containing 10–20% FBS. Survival of SBT-E1 after short-term cryostorage was 70.8±12.8%. The species of origin was confirmed by sequencing a region of the mitochondrial gene for cytochrome c oxidase, subunit I (cox1). The SBT-E1 karyotype, with a modal chromosome number of 2n=46, differs from the constitutional karyotype of the parent species (2n=48), with the apparent loss of either whole or partial chromosomes and the presence of small marker chromosomes. The SBT-E1 cell line represents the first reported cell line from any species of tuna.
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