Genes encoding s-triazine degradation are plasmid-borne in Klebsiella pneumoniae strain 99
1997
Karns, J.S. | Eaton, R.W.
Klebsiella pneumoniae strain 99 degrades the s-triazine compound ammelide through cyanuric acid and biuret to yield urea, carbon dioxide, and ammonia. The urea and ammonia formed from the degradation of ammelide or cyanuric acid are utilized as sources of nitrogen for growth of the organism. When plasmids of the IncI alpha incompatibility group were transferred into K. pneumoniae strain 99, the ability to degrade s-triazine compounds was lost at high frequency. Analysis of the plasmid profiles of s-triazine+ and s-triazine- derivatives of strain 99 indicated that the largest of the at least five plasmids detected in this organism carries the genes encoding the s-triazine degradation pathway. Conjugal transfer of this plasmid from wild-type strain 99 into a type strain of Klebsiella planticola (ATCC 33531) resulted in exconjugants able to utilize ammelide or cyanuric acid as nitrogen sources. Thus, the genes required for s-triazine degradation are present on a large IncI alpha plasmid in K. pneumoniae strain 99.
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